پیشرفت‌ها در روش‌های زیست‌شناسی مولکولی و ساختاری پروتئین ۲۰۲۲
Advances in Protein Molecular and Structural Biology Methods 2022

1. Title of Book

2. Cover image

3. Title page

4. Table of Contents

5. Copyright

6. Dedicated to

7. Contributors

8. About the Editors

9. Foreword

10. Preface

11. Chapter 1 Strategies to improve the expression and solubility of recombinant proteins in E. coli

12. Chapter 2 Advances in heterologous protein expression strategies in yeast and insect systems

13. Chapter 3 Methods for transient expression and purification of monoclonal antibodies in mammalian cells

14. Chapter 4 Methods for recombinant production and purification of intrinsically disordered proteins

15. Chapter 5 Methods to determine the oligomeric structure of proteins

16. Chapter 6 Multimodal methods to study protein aggregation and fibrillation

17. Chapter 7 Experimental methods to study the thermodynamics of protein–protein interactions

18. Chapter 8 Experimental methods to study the kinetics of protein–protein interactions

19. Chapter 9 Computational techniques for studying protein-protein interactions

20. Chapter 10 Experimental methods to study protein–nucleic acid interactions

21. Chapter 11 Advanced computational tools for quantitative analysis of protein–nucleic acid interfaces

22. Chapter 12 Experimental techniques to study protein dynamics and conformations

23. Chapter 13 Computational techniques to study protein dynamics and conformations

24. Chapter 14 Application of circular dichroism spectroscopy in studying protein folding, stability, and interaction

25. Chapter 15 Studying protein-folding dynamics using single-molecule fluorescence methods

26. Chapter 16 Advances in liquid-state NMR spectroscopy to study the structure, function, and dynamics of biomacromolecules

27. Chapter 17 In-cell NMR spectroscopy: A tool to study cellular structure biology

28. Chapter 18 Current trends in membrane protein crystallography

29. Chapter 19 Advances in sample preparation and data processing for single-particle cryo-electron microscopy

30. Chapter 20 Advanced mass spectrometry-based methods for protein molecular-structural biologists

31. Chapter 21 Developments, advancements, and contributions of mass spectrometry in omics technologies

32. Chapter 22 Role of structural biology methods in drug discovery

33. Chapter 23 Prediction, validation, and analysis of protein structures: A beginner’s guide

34. Chapter 24 Advances in structure-based virtual screening for drug discovery

35. Chapter 25 Methods and applications of machine learning in structure-based drug discovery

36. Chapter 26 Molecular dynamics simulations: Principles, methods, and applications in protein conformational dynamics

37. Chapter 27 Applications of molecular dynamics simulations in drug discovery

38. Chapter 28 Envisaging the conformational space of proteins by coupling machine learning and molecular dynamics

39. Chapter 29 Immunoinformatics and reverse vaccinology methods to design peptide-based vaccines

40. Chapter 30 Computational methods to study intrinsically disordered proteins

41. Chapter 31 Experimental methods to study intrinsically disordered proteins

42. Chapter 32 Analysis of structure and dynamics of intrinsically disordered regions in proteins using solution NMR methods

43. Chapter 33 Methods to study the effect of solution variables on the conformational dynamics of intrinsically disordered proteins

44. Chapter 34 Molecular simulations to study IDP-IDP interactions and their complexes

45. Chapter 35 Exploring large-scale protein function using systematic mutant analysis

46. Chapter 36 Approaches and methods to study cell signaling: Linguistics of cellular communication

47. Chapter 37 Methods to study systems biology of signaling networks: A case study of NSCLC

48. Chapter 38 Advancements in the analysis of protein post-translational modifications

49. Chapter 39 Protein engineering: Methods and applications

50. Chapter 40 Designer 3D-DNA nanodevices: Structures, functions, and cellular applications

51. Index