الکتروفورز ۸۱: روش های پیشرفته، کاربردهای بیوشیمیایی و بالینی. مجموعه مقالات سومین کنفرانس بین المللی الکتروفورز، چارلستون، کارولینای جنوبی، ۷ تا ۱۰ آوریل ۱۹۸۱. [برگزار شده در conjunction با اولین جلسه سالانه انجمن الکتروفورز] ۲۰۱۹
Electrophoresis ’81: Advanced methods, biochemical and clinical applications. Proceedings of the Third International Conference on Electrophoresis, Charleston, SC, April 7–10, 1981. [held in conjunction with the first annual meeting of the Electrophoresis Society] 2019

1. PREFACE

2. CONTENTS

3. SECTION I. THEORY AND METHODS

4. ON THE PORE SIZE AND SHAPE OF HYDROPHILLIC GELS FOR ELECTROPHORETIC ANALYSIS

5. GENERALIZATION OF THE DEBYE-HUCKEL THEORY TO MACROION SOLUTIONS OF FINITE SIZE AND CONCENTRATION AS APPLIED TO THEIR ELECTROPHORETIC MOBILITY AND TRANSLATIONAL DIFFUSION COEFFICIENT

6. CALCULATION OF THE THERMODYNAMIC CONSTANTS OF CONCANAVALIN A - CARBOHYDRATE INTERACTIONS BY MEANS OF AFFINITY ELECTROPHORESIS

7. HIGH RESOLUTION ELECTROPHORESIS OF PROTEINS IN SDS POLYACRYLAMIDE GELS

8. CONVENIENT PROCEDURES FOR SDS AND CONVENTIONAL DISC ELECTROPHORESIS

9. THE DESIGN AND APPLICATIONS OF A UNIVERSAL GEL ELECTROPHORESIS APPARATUS

10. A SIMPLE METHOD FOR CASTING POLYACRYLAMIDE GELS OF VARIOUS THICKNESS AND SIZE FOR FLAT-BED ISOELECTRIC FOCUSING

11. ISOELECTRIC FOCUSING ON CELLULOSIC MEMBRANES

12. A METHOD FOR PHENOTYPING OF ALPHA-1-ANTITRYPSIN VARIANTS USING SEPARATOR ISOELECTRIC FOCUSING ON AGAROSE

13. QUANTIFICATION OF PROTEINS WITH ZONE IMMUNOELECTROPHORESIS

14. SPECIFIC AND EFFICIENT PROCEDURES FOR QUANTIFICATION OF PROTEINS SEPARATED IN GEL ELECTROPHORETIC METHODS USING ZONE IMMUNOELECTROPHORETIC ASSAY (ZIA)

15. A SIMPLIFIED POLYACRYLAMIDE DENSITY GRADIENT GEL ELECTROPHORESIS WITH A NEW STAINING METHOD, ELECTROSTAINING

16. ENHANCEMENT METHODS IN THE LOCALIZATION OF PROTEINS FOLLOWING ELECTROPHORESIS OR ISOELECTRIC FOCUSING

17. ONE AND TWO-DIMENSIONAL MICROELECTROPHORESIS AND STAINING OF PROTEINS WITH A SILVER METHOD

18. "COLD FOCUS" ISOELECTRIC FOCUSING OF SMALL SAMPLES OF CEREBROSPINAL FLUID

19. A UNIQUE SILVER STAINING PROCEDURE FOR COLOUR CHARACTERIZSATION OF POLYPEPTIDES

20. AFFINITY-IMMUNODELETION (AID) ISOELECTRIC FOCUSING ON ULTRATHIN GELS APPLIED TO THE IDENTIFICATION OF SWEAT, SALIVA AND BLOOD PROTEINS USING SILVER-DIAMINE STAINING

21. ULTRATHIN LAYER ISOELECTRIC FOCUSING IN 20-50 um POLYACRYLAMIDE GELS: COMPARISON OF CONVENTIONAL AND MINIATURE SYSTEMS

22. ULTRATHIN LAYER PAGIF: A COST BENEFIT ANALYSIS OF THE FOCUSING DISTANCE

23. ISOELECTRIC FOCUSING IN AGAROSE GELS

24. SEAPREP^TM 15/45.. A NEW AGAROSE WITH LOW GELLING AND REMELTING PROPERTIES FOR PREPARATIVE ELECTROPHORESIS

25. AGAROSE GEL ELECTROENDOSMOSIS: ITS ENHANCEMENT, REDUCTION AND PRACTICAL RAMIFICATIONS

26. ENHANCEMENT OF COMPLEX PATTERNS IN ISOELECTRIC FOCUSING WITH IMPROVED ISOGEL PERFORMANCE

27. THE SIEVING OF VIRUSES AND VIRAL CAPSIDS DURING AGAROSE GEL ELECTROPHORESIS

28. NEW ELECTROPHORETIC TECHNIQUES FOR THE SEPARATION AND CHARACTERIZATION OF POLIO VIRUS PROTEINS

29. SECTION II. HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS

30. SDS-GEL GRADIENT ELECTROPHORESIS, ISOELECTRIC FOCUSING AND HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS IN HORIZONTAL, ULTRATHIN-LAYER POLYACRYLAMIDE GELS

31. TWO-DIMENSIONAL ELECTROPHORESIS ON CELLULOSE ACETATE MEMBRANES

32. ISOELECTRIC FOCUSING, SDS GEL GRADIENT ELECTROPHORESIS AND TWO-DIMENSIONAL ELECTROPHORESIS OF TROPICAL AND EUROPEAN LEGUME PROTEINS IN ULTRATHIN POLYACRYLAMIDE LAYERS

33. HIGH RESOLUTION POLYPEPTIDE MAPPING OF HUMAN HAIR ROOTS FROM HEALTHY INDIVIDUALS AND PATIENTS WITH GENETIC DEFECTS

34. ANALYSIS OF CULTURED SKIN FIBROBLASTS FROM PATIENTS WITH DUCHENNE MUSCULAR DYSTROPHY USING ELECTROPHORETIC TECHNIQUES

35. STUDIES OF GENE EXPRESSION IN HUMAN LYMPHOCYTES USING HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS

36. ISOELECTRIC FOCUSING AND TWO DIMENSIONAL MAPS OF NORMAL AND CYSTIC FIBROSIS SALIVA

37. ANALYSIS OF HUMAN AMNIOTIC FLUID PROTEINS BY TWODIMENSIONAL ELECTROPHORESIS

38. QUANTITATIVE TWO-DIMENSIONAL ELECTROPHORESIS AS A SCREEN FOR GENETIC DISEASE MARKERS

39. A QUANTITATIVE TWO-DIMENSIONAL ELECTROPHORETIC SURVEY OF PROTEINS AFFECTED BY CHROMOSOME 21

40. MULTISPECTRAL DIGITAL IMAGE ANALYSIS OF COLOR TWODIMENSIONAL ELECTROPHORETOGRAMS

41. A COMPUTERIZED SYSTEM FOR MATCHING AND STRETCHING TWO-DIMENSIONAL GEL PATTERNS REPRESENTED BY PARAMETER LISTS

42. GELLAB: MULTIPLE 2D ELECTROPHORETIC GEL ANALYSIS

43. IMPLICIT MODELING OF SPOTS FOR THE EVALUATION OF TWO-DIMENSIONAL ELECTROPHORETOGRAMS

44. SECTION III. BIOMEDICAL AND BIOLOGICAL APPLICATIONS

45. EVALUATION OF IMMUNOGLOBULIN DIVERSITY

46. STUDIES OF ANTIBODY CLONOTYPE PATTERNS IN RABBIT ANTIMICROCOCCAL SERA BY ANALYTICAL ISOELECTRIC FOCUSING IN AGAROSE GELS

47. MONOCLONAL GAMMOPATHY WITH FREE LIGHT CHAINS

48. ISOELECTRIC FOCUSING OF IMMUNOGLOBULINS IN ULTRATHIN LAYERS OF AGAROSE

49. ELECTROPHORETIC ABNORMALITIES OF HIGH DENSITY LIPOPROTEINS IN LIVER DISEASES

50. SDS-PAGE OF URINARY PROTEINS: CORRELATION OF DIFFERENT PROTEINS TO RENAL CLEARANCE AND TO RENAL AND EXTRARENAL DISEASES

51. HEPATIC IMPLICATIONS IN THE METABOLISM OF HUMAN SALIVARY AMYLASE ISOENZYME

52. POSTTRANSLATIONAL MODIFICATION OF PANCREATIC AMYLASE IN ACUTE PANCREATITIS: POSSIBLE ROLE OF PANCREATIC DEAMIDASE

53. EFFECTS OF DIALYZABLE LEUKOCYTE EXTRACTS WITH TRANSFER FACTOR ACTIVITY ON LEUKOCYTE MIGRATION IN VITRO: VI. STUDIES ON THE PRIMARY STRUCTURE OF TRANSFER FACTOR

54. CHARACTERIZATION OF HORSE PLASMA PROTEINS BY SPECTROPHOTOMETRY AND ISOELECTRIC FOCUSING

55. ALPHA-1-ANTITRYPSIN DEFICIENCY AND DISEASE

56. RARE TYPES OF ALPHA-1-ANTITRYPSIN ASSOCIATED WITH DEFICIENCY

57. ISOLATION AND CHARACTERIZATION OF AN ALPHA-1"ANTITRYPSIN -RELATED GLYCOPROTEIN FROM HUMAN LIVER

58. CK-MB BY AN IMPROVED ELECTROPHORESIS METHOD

59. DETECTION AND CHARACTERIZATION OF CYSTIC FIBROSIS PROTEIN EMPLOYING ISOELECTRIC FOCUSING AND IMMUNOELECTROPHORETIC TECHNIQUES

60. IMMUNOFIXATION-AGAROSE ISOELECTRIC FOCUSING TECHNIQUES FOR SCREENING GLIAL TUMOR CELL CULTURES FOR GLIAL MARKERS

61. AGAROSE ISOELECTRIC FOCUSING AND RELATED IMMUNOCHEMICAL TECHNIQUES IN THE ANALYSIS OF SERUM GC-GLOBULIN

62. ISOELECTRIC FOCUSING IN FORENSIC SEROLOGY

63. ISOELECTRIC FOCUSING OF THE COMMON TRANSFERRIN0 ALLELE SUBTYPES

64. ISOELECTRIC FOCUSING OF HAIR PROTEINS

65. ISOENZYME TYPING OF DRIED BLOODSTAINS USING MYLAR BACKED CELLULOSE ACETATE MEMBRANES

66. CHANGES IN THE ELECTROPHORETIC PATTERNS OF STRUCTURAL PROTEINS AND ENZYMES OF HALIBUT (HIPPOGLOSSUS HIPPOGLOSSUS L.) MUSCLE AFTER PROLONGED (TWENTY-FIVE YEARS" FROZEN STORAGE

67. ELECTROPHORETIC BEHAVIOR OF CROSSLINKED DNA

68. ELECTROPHORETIC MIGRATION OF FORM IV-DNA AND TOPOISOMERASE MONITORING

69. PROPERTIES OF NUCLEIC ACIDS PURIFIED FROM AGAROSE

70. FRACTIONATION OF CONCATEMERIC DNA OF BACTERIOPHAGE T7 BY AGAROSE GEL ELECTROPHORESIS

71. DNA SEQUENCING ON VERY THIN (0,2mm) GELS

72. THE DETECTION OF HUMAN INTRACELLULAR DEXOXYRIBONUCLEASE ACTIVITIES USING ONE DIMENSIONAL POLYNUCLEOTIDE— POLYACRYLAMIDE GEL ELECTROPHORESIS

73. RECENT DEVELOPMENTS IN TITRATION CURVES OF PROTEINS BY ISOELECTRIC FOCUSING-ELECTROPHORESIS

74. A STUDY OF THE STRUCTURAL CONVERSIONS OF TWO CARBOXYL PROTEINASES EMPLOYING ELECTROPHORESIS ACROSS A pH GRADIENT

75. ELASTASE DETECTION IN ELECTROPHORETOGRAMS OF COMPLEX ANTIGEN MIXTURES

76. POSITIVE PERIODIC ACID-SCHIFF STAINING OF PROTEOLYTIC ENZYMES FOLLOWING ZONE ELECTROPHORESIS IN POLYACRYLAMIDE GEL

77. ELECTROPHORETIC AND STAIN METHOD FOR PYRUVATE KINASE ISOENZYME PATTERNS

78. ISOENZYMES OF SUPEROXIDE DISMUTASE IN SERRATIA MARCESCENS

79. ISOENZYMES OF SUPEROXIDE DISMUTASE IN LIVER FROM LACERTA STEHLINII

80. ELECTROPHORETIC MULTIPLICITIES OF γ-GLUTAMYLTRANSFERASES IN HUMAN SERUM AND THEIR CHARACTERIZATION BY AFFINITY CHROMATOGRAPHY

81. ISOAMYLASE BY ISOELECTRIC FOCUSING ON MODIFIED CELLULOSE ACETATE

82. MULTIPLE FORMS OF TREHALASE FROM AGING SOROCARPS OF THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM

83. PURIFICATION OF GRANULOCYTE COLONY STIMULATING FACTOR MONITORED BY ULTRATHIN LAYER ISOELECTRIC FOCUSING

84. PROTEOLYTIC DIGESTION OF THE M PROTEIN OF INFLUENZA A VIRUSES AND ISOELECTRIC FOCUSING OF ITS PEPTIDES

85. SECTION IV. ISOTACHOPHORESIS AND FREE FLOW ELECTROPHORESIS

86. QUANTITATIVE ANALYSIS IN ISOTACHOPHORESIS

87. THE ANALYSIS OF HUMAN SERUM PROTEINS BY CAPILLARY ISOTACHOPHORESIS

88. ISOTACHOPHORETIC ASSESSMENT OF ENZYME IMMUNOGLOBULIN CONJUGATES USED IN ENZYME IMMUNOASSAY

89. ANALYTICAL CAPILLARY ISOTACHOPHORESIS APPLIED TO THE STUDY OF NUCLEOTIDE-DEPENDENT ENZYMATIC PROCESSES

90. CONDITIONS FOR THE SEPARATION OF ADENINE NUCLEOTIDES AND THEIR 3 ^-PHOSPHATE DERIVATIVES IN ANALYTICAL CAPILLARY ISOTACHOPHORESIS

91. ISOTACHOPHORETIC ANALYSIS OF HIGH DENSITY LIPOPROTEINS

92. CAPILLARY ISOTACHOPHORESIS AS AN ANALYTICAL MONITOR DURING THE SYNTHESIS OF THE C-TERMINAL PENTAPEPTIDE OF BOMBININ

93. AN ALTERNATIVE OUTLOOK ON ELECTROKINETIC CELL SEPARATIONS

94. CHARACTERIZATION OF THE SURFACE PHENOTYPE OF ELECTROPHORECTICALLY FRACTIONATED MOUSE LYMPHOCYTES BY FLOW MICROFLUOREMETRY ANALYSIS

95. EVALUATION OF THE ELECTROPHORETIC SEPARABILITY OF TRYPANOSOMA CRUZI PARASITE STAGES

96. SEPARATION AND CHARACTERIZATION OF HUMAN BONE MARROW CELLS BY DENSITY GRADIENT ELECTROPHORESIS

97. FREE FLOW ELECTROPHORESIS IN MALARIA RESEARCH

98. LIBERATION OF INTRAERYTHROCYTIC PARASITES (PLASMODIUM VINCKEI, PLASMODIUM BERGHEI) FROM ERYTHROCYTES AND ELECTROPHORETIC SEPARATION OF FREE PARASITES FROM HOST CELL CONSTITUENTS

99. FREE-FLOW ELECTROPHORESIS SEPARATIONS OF FREE PARASITES (PLASMODIUM FALCIPARUM)ACCORDING TO STAGES

100. FREE-FLOW ELECTROPHORESIS ISOLATION OF WHOLE BODY PLASMODIUM BERGHEI SPOROZOITES

101. SEPARATION OF FUNCTIONING MAMMALIAN CELLS BY DENSITYGRADIENT ELECTROPHORESIS

102. REVIEW OF THE NASA ELECTROPHORESIS PROGRAM

103. NUMERICAL ANALYSIS OF CONTINUOUS FLOW ELECTROPHORESIS

104. HIGH-RESOLUTION CONTINUOUS-FLOW ELECTROPHORESIS IN THE REDUCED GRAVITY ENVIRONMENT

105. APPLICATION OF THE AUTOMATED ELECTROPHORESIS MICROSCOPE SYSTEM TO VARIOUS BIOLOGICAL TESTS

106. OPERATIONAL PARAMETERS FOR CONTINUOUS FLOW ELECTROPHORESIS OF CELLS

107. REDUCTION OF DROPLET FORMATION AND SEDIMENTATION OF FIXED ERYTHROCYTES IN STATIONARY AND FLOWING SYSTEMS

108. DESIGN CONSIDERATIONS OF A THERMALLY STABILIZED CONTINUOUS FLOW ELECTROPHORESIS CHAMBER

109. THE SHEEP ERYTHROCYTES ELECTROPHORETIC MOBILITY: EFFECT OF SUPERNATANTS OF LYMPHOCYTES STIMULATED WITH VARIOUS IMMUNOPOTENTIATORS

110. ELECTRICAL INSULATION OF ELECTROPHORETIC FLOWS AND A "RAIN BOX"

111. Author Index

112. Subject Index